Beilstein J. Nanotechnol.2020,11, 1381–1393, doi:10.3762/bjnano.11.122
on the Care and Use of Laboratory Animals 86/609/EEC) and the experimental protocols were approved by the Animal Care and Use Committee of the Palladin Institute of Biochemistry (Protocol from 19/09-2016). Ten animals were used in experiments analyzing ʟ-[14C]glutamate uptake, its extracellularlevel
µg/mL changed the fluorescence intensity of rhodamine 6G, which was associated with a decrease in transparency of the analyzed solution. In the fluorescence experiments, a γ-Fe2O3/synaptosomal protein ratio similar to that used in the experiments regarding uptake and extracellularlevel of ʟ-[14C
in uptake and an increase in the extracellularlevel of glutamate in nerve cells.
It should be noted that different concentrations of γ-Fe2O3 nanoparticles were used in the experiments. Nanoparticles within a concentration range of 0.75–3 mg/mL were applied in ʟ-[14C]glutamate adsorption experiments
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Figure 1:
TEM micrograph of γ-Fe2O3 nanoparticles.
Beilstein J. Nanotechnol.2014,5, 778–788, doi:10.3762/bjnano.5.90
characteristics of the glutamatergic neurotransmission were analysed. Using radiolabeled L-[14C]glutamate, it was shown that D-mannose-coated γ-Fe2O3 nanoparticles did not affect high-affinity Na+-dependent uptake, tonic release and the extracellularlevel of L-[14C]glutamate in isolated rat brain nerve terminals
field using D-mannose-coated γ-Fe2O3 nanoparticles, while the key characteristics of glutamatergic neurotransmission are not affected. In other words, functionally active synaptosomes labeled with D-mannose-coated γ-Fe2O3 nanoparticles were obtained.
Keywords: extracellularlevel; γ-Fe2O3; glutamate
normal brain functioning. Impaired glutamate homeostasis causes neuronal dysfunction and contributes to the pathogenesis of major neurological disorders. For normal brain functioning, a low extracellularlevel of glutamate should be maintained between episodes of exocytotic release, thereby preventing
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Figure 1:
Transmission electron micrographs of (a) neat and (b) D-mannose-coated γ-Fe2O3 nanoparticles.